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Keywords:

  • directed evolution;
  • DNA structures;
  • enzyme catalysis;
  • enzymes;
  • selection
Thumbnail image of graphical abstract

Running in reverse: A set of enzymes selected according to their RNA-dependent DNA polymerase activity from a library of more than 107 mutant enzymes has been characterized. The product was specifically bound to catalytically active proteins displayed on filamentous bacteriophage. The selected variants have a catalytic efficiency for reverse transcription that is two orders of magnitude higher.