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Control of the Yeast Cell Cycle with a Photocleavable α-Factor Analogue


  • L.P. gratefully acknowledges the NIH for a Ruth L. Kirschstein Individual Postdoctoral Fellowship (F32). S.J.K. is a Scholar of the Leukemia and Lymphoma Society.


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Under arrest: Photodegradation of a synthetic yeast α-factor analogue releases yeast from mating-factor-induced arrest into the cell cycle. This peptide has sufficient activity to arrest cells in G1 at reasonable doses and it can be specifically degraded through exposure to UV light at a wavelength that is “safe” for most cells. FITC-A=absorption of a fluorescein isothiocyanate analogue.