Communication

Tissue Imaging at Atmospheric Pressure Using Desorption Electrospray Ionization (DESI) Mass Spectrometry†

  1. Justin M. Wiseman,
  2. Demian R. Ifa Dr.,
  3. Qingyu Song,
  4. R. Graham Cooks Prof.

Article first published online: 26 SEP 2006

DOI: 10.1002/anie.200602449

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 45, Issue 43, pages 7188–7192, November 6, 2006

Additional Information

How to Cite

Wiseman, J. M., Ifa, D. R., Song, Q. and Cooks, R. G. (2006), Tissue Imaging at Atmospheric Pressure Using Desorption Electrospray Ionization (DESI) Mass Spectrometry. Angewandte Chemie International Edition, 45: 7188–7192. doi: 10.1002/anie.200602449

Author Information

  1. Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA, Fax: (+1) 765-494-0239 http://www.chem.purdue.edu/cooks/

  1. †

    This work was supported by Prosolia Inc. (Indianapolis, IN) and the Office of Naval Research, and performed in the Bindley Biosciences Center. The authors gratefully thank Candice Kissinger, James Woods, and Peter Kissinger (Bioanalytical Systems Inc., West Lafayette, IN) for advice and for providing rat brain specimens for these studies. The authors also thank Shannon Cornett (Vanderbilt University) for information on data conversion. In addition, we thank Dominik Lenz (Purdue University, Bindley Bioscience Center) for providing optical images of rat brain sections. Frontispiece artwork by D.R.I. and J.M.W.

Publication History

  1. Issue published online: 30 OCT 2006
  2. Article first published online: 26 SEP 2006
  3. Manuscript Received: 18 JUN 2006

Funded by

  • Prosolia Inc. (Indianapolis, IN)
  • Office of Naval Research

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Keywords:

  • lipids;
  • mass spectrometry;
  • microscopy;
  • phospholipids;
  • surface analysis

Graphical Abstract

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Images of the masses: Two-dimensional molecular imaging of intact rat brain tissue under ambient conditions was achieved by using the mass spectrometric technique of desorption electrospray ionization (DESI). The distributions of specific lipids, such as sulfatide 24:1, reveal distinctive subanatomical features of the rat brain (cc=corpus callosum; aca=anterior part of anterior commissure) with spatial resolution of less than 500 μm.

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