We thank the Fonds der Chemischen Industrie and the German-Israeli Project Cooperation (DIP) for support.
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Communication
Iterative Saturation Mutagenesis on the Basis of B Factors as a Strategy for Increasing Protein Thermostability†
Article first published online: 31 OCT 2006
DOI: 10.1002/anie.200602795
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 45, Issue 46, pages 7745–7751, November 27, 2006
Additional Information
How to Cite
Reetz, M. T., Carballeira, J. D. and Vogel, A. (2006), Iterative Saturation Mutagenesis on the Basis of B Factors as a Strategy for Increasing Protein Thermostability. Angew. Chem. Int. Ed., 45: 7745–7751. doi: 10.1002/anie.200602795
- †
Publication History
- Issue published online: 20 NOV 2006
- Article first published online: 31 OCT 2006
- Manuscript Revised: 18 AUG 2006
- Manuscript Received: 13 JUL 2006
Funded by
- Fonds der Chemischen Industrie
- German-Israeli Project Cooperation (DIP)
- Abstract
- Article
- References
- Cited By
Keywords:
- biotransformation;
- directed evolution;
- enzymes;
- protein engineering;
- protein stability
Efficiency counts: A new method of directed evolution for increasing the thermostability of proteins saves time and effort. In an illustrative study, iterative cycles of saturation mutagenesis were performed at those sites in a lipase that display high B factors.