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Keywords:

  • enzymes;
  • metalloenzymes;
  • nitrogen oxides;
  • Raman spectroscopy;
  • structure elucidation
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NO laughing matter: The enzymatic production of laughing gas (N2O) from two molecules of NO requires the formation of the N[BOND]N bond and the subsequent cleavage of the N[BOND]OH bond. Resonance Raman spectroscopic studies have now provided evidence for the formation of the key intermediate, the hyponitrite species (see the optimized molecular structure), in the heme a3 Fe[BOND]CuB binuclear center of ba3-oxidoreductase from Thermus thermophilus.