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Fast High-Resolution Protein Structure Determination by Using Unassigned NMR Data

Authors

  • Jegannath Korukottu,

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • Monika Bayrhuber,

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • Pierre Montaville Dr.,

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • Vinesh Vijayan,

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • Young-Sang Jung Dr.,

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • Stefan Becker Dr.,

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • Markus Zweckstetter Dr.

    1. Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-2202
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  • We thank Christian Griesinger for useful discussions, Karin Giller and Kamila Sabagh for technical assistance, and Baldomero M. Olivera for the cDNA clones of Conk-S1 and Conk-S2. This work was supported by the Max Planck Society. M.Z. is the recipient of a DFG Emmy Noether fellowship (ZW 71/1-5).

Abstract

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The major bottleneck of rapid NMR-based structure determination is data analysis. The solution to this problem is a method that enables automatic high-resolution protein-structure determination from unassigned, experimental NMR data without manual intervention in less than 24 h.

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