Chemiluminescent Energy-Transfer Cassettes Based on Fluorescein and Nile Red


  • We thank Prof. Robin M. Hochstrasser (University of Pennsylvania) for helpful discussions and the National Institutes of Health (GM72041) and The Robert A. Welch Foundation for providing financial support.


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The long and the short of it: Luminol chemiluminesces with a beautiful blue color; however, to be useful for biotechnological applications, the emission must be shifted to much longer wavelengths. Energy-transfer cassettes like that shown in the picture provide one solution.