Is the Ferric Hydroperoxy Species Responsible for Sulfur Oxidation in Cytochrome P450s?


  • The authors are grateful to Prof. S. Chapman (University of Edinburgh) for the P450BM3 clones used in this work.


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Not the ferryl oxidant? The oxidation of thiafatty acids by P450BM3 (CYP102A1) was shown to be consistent with oxidation by the ferric hydroperoxy species (Cpd 0) rather than the expected ferryl species (Cpd 1). This is the first indication that sulfur oxidation in a wildtype P450 occurs via the hydroperoxy species.