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Keywords:

  • aggregation;
  • assays;
  • charge interaction;
  • nanoparticles;
  • phosphatases
Thumbnail image of graphical abstract

To change your colors: A colorimetric enzyme assay based on peptide-induced aggregation of gold nanoparticles (Au-NPs) has been developed to sense phosphatase activity. Once the phosphate group is eliminated by alkaline phosphatase, peptide-induced Au-NP aggregation is triggered, resulting in a significant change in absorbance at 650 nm.