These authors contributed equally to this work.
Sensing Phosphatase Activity by Using Gold Nanoparticles†
Article first published online: 4 DEC 2006
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 46, Issue 5, pages 707–709, January 22, 2007
How to Cite
Choi, Y., Ho, N.-H. and Tung, C.-H. (2007), Sensing Phosphatase Activity by Using Gold Nanoparticles. Angew. Chem. Int. Ed., 46: 707–709. doi: 10.1002/anie.200603735
This research was supported by NIH grants P50-CA86355 and RO1 CA99385.
- Issue published online: 12 JAN 2007
- Article first published online: 4 DEC 2006
- Manuscript Received: 12 SEP 2006
- NIH. Grant Numbers: P50-CA86355, RO1 CA99385
- charge interaction;
To change your colors: A colorimetric enzyme assay based on peptide-induced aggregation of gold nanoparticles (Au-NPs) has been developed to sense phosphatase activity. Once the phosphate group is eliminated by alkaline phosphatase, peptide-induced Au-NP aggregation is triggered, resulting in a significant change in absorbance at 650 nm.