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Sensing Phosphatase Activity by Using Gold Nanoparticles

Authors

  • Yongdoo Choi Dr.,

    1. Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Building 149, 13th street, Charlestown, MA 02129, USA, Fax: (+1) 617-726-5708
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    • These authors contributed equally to this work.

  • Nan-Hui Ho Dr.,

    1. Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Building 149, 13th street, Charlestown, MA 02129, USA, Fax: (+1) 617-726-5708
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    • These authors contributed equally to this work.

  • Ching-Hsuan Tung Prof. Dr.

    1. Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Building 149, 13th street, Charlestown, MA 02129, USA, Fax: (+1) 617-726-5708
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  • This research was supported by NIH grants P50-CA86355 and RO1 CA99385.

Abstract

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To change your colors: A colorimetric enzyme assay based on peptide-induced aggregation of gold nanoparticles (Au-NPs) has been developed to sense phosphatase activity. Once the phosphate group is eliminated by alkaline phosphatase, peptide-induced Au-NP aggregation is triggered, resulting in a significant change in absorbance at 650 nm.

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