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A Mild Chemically Cleavable Linker System for Functional Proteomic Applications

Authors

  • Steven H. L. Verhelst Dr.,

    1. Department of Pathology and Department of Microbiology & Immunology, Stanford School of Medicine, 300 Pasteur Dr., Stanford, CA 94305, USA, Fax: (+1) 650-725-7424
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  • Marko Fonović Dr.,

    1. Department of Pathology and Department of Microbiology & Immunology, Stanford School of Medicine, 300 Pasteur Dr., Stanford, CA 94305, USA, Fax: (+1) 650-725-7424
    2. Department of Biochemistry and Molecular Biology, Josef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
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  • Matthew Bogyo Dr.

    1. Department of Pathology and Department of Microbiology & Immunology, Stanford School of Medicine, 300 Pasteur Dr., Stanford, CA 94305, USA, Fax: (+1) 650-725-7424
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  • This work was financially supported by a National Technology Center for Networks and Pathways grant (U54 RR020843) and a NIH grant R01-EB005011 (to M.B.) and a postdoctoral fellowship from the Netherlands Organization for Scientific Research (to S.V.).

Abstract

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An amicable split: Small-molecule probes can be applied for the enrichment of specific protein targets from a complex proteome. A cleavable linker system, which prevents the release of unwanted, nonspecifically bound proteins, can be used for a very mild and highly selective cleavage of probe-labeled proteins.

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