We thank the NIH AIDS Reagent Program for providing the three human T cell lines and two MAGI cell lines. This research was supported in part by the Ludwig Translational Research Program at Stanford University and a grant from the NIH/NCI Center for Cancer Nanotechnology Excellence (CCNE). siRNA=short interfering RNA.
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Communication
siRNA Delivery into Human T Cells and Primary Cells with Carbon-Nanotube Transporters†
Article first published online: 9 FEB 2007
DOI: 10.1002/anie.200604295
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Liu, Z., Winters, M., Holodniy, M. and Dai, H. (2007), siRNA Delivery into Human T Cells and Primary Cells with Carbon-Nanotube Transporters. Angew. Chem. Int. Ed., 46: 2023–2027. doi: 10.1002/anie.200604295
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Publication History
- Issue published online: 2 MAR 2007
- Article first published online: 9 FEB 2007
- Manuscript Received: 19 OCT 2006
Funded by
- Ludwig Translational Research Program
- NIH/NCI Center for Cancer Nanotechnology Excellence (CCNE)
Keywords:
- carbon nanotubes;
- drug delivery;
- nanobiotechnology;
- RNA interference;
- T cells
Special delivery: Functionalized single-walled nanotubes (SWNT) can be used as molecular transporters to shuttle short interfering RNA (siRNA) into human T cells and primary cells and silence the expression of HIV-specific cell-surface receptors and coreceptors (see picture; scale bars 40 μm). This silencing effect, known to block HIV viral entry and reduce infection, is superior to that observed with conventional liposome-based nonviral delivery agents.