Cross-Linking Yield Variation of a Potent Matrix Metalloproteinase Photoaffinity Probe and Consequences for Functional Proteomics

Authors

  • Arnaud David,

    1. CEA, Institut de Biologie et des Technologies de Saclay (iBiTecS), Service d'Ingénierie Moléculaire des Proéines (SIMOPRO), 91191 Gif/Yvette Cedex, France, Fax: (+33) 1-6908-9071
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    • These authors contributed equally to this work.

  • David Steer Dr.,

    1. CEA, Institut de Biologie et des Technologies de Saclay (iBiTecS), Service d'Ingénierie Moléculaire des Proéines (SIMOPRO), 91191 Gif/Yvette Cedex, France, Fax: (+33) 1-6908-9071
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    • These authors contributed equally to this work.

  • Sarah Bregant Dr.,

    1. CEA, Institut de Biologie et des Technologies de Saclay (iBiTecS), Service d'Ingénierie Moléculaire des Proéines (SIMOPRO), 91191 Gif/Yvette Cedex, France, Fax: (+33) 1-6908-9071
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  • Laurent Devel Dr.,

    1. CEA, Institut de Biologie et des Technologies de Saclay (iBiTecS), Service d'Ingénierie Moléculaire des Proéines (SIMOPRO), 91191 Gif/Yvette Cedex, France, Fax: (+33) 1-6908-9071
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  • Anastasios Makaritis Dr.,

    1. Laboratory of Organic Chemistry, University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece
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  • Fabrice Beau,

    1. CEA, Institut de Biologie et des Technologies de Saclay (iBiTecS), Service d'Ingénierie Moléculaire des Proéines (SIMOPRO), 91191 Gif/Yvette Cedex, France, Fax: (+33) 1-6908-9071
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  • Athanasios Yiotakis Prof. Dr.,

    1. Laboratory of Organic Chemistry, University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece
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  • Vincent Dive Dr.

    1. CEA, Institut de Biologie et des Technologies de Saclay (iBiTecS), Service d'Ingénierie Moléculaire des Proéines (SIMOPRO), 91191 Gif/Yvette Cedex, France, Fax: (+33) 1-6908-9071
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  • This work was supported by funds from FP6RDT (LSHC-CT-2003-503297).

Abstract

original image

Probing proteinases: A radioactive photoaffinity probe exhibiting subnanomolar potency towards matrix metalloproteinases (MMPs) has been developed (see structure). High sensitivity in the detection of particular MMPs has been obtained; however, high variation in the cross-linking yield of MMPs with this probe may limit its ability to detect all MMP active forms in biological samples. This result suggests that a cocktail of optimized probes should be developed.

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