These authors contributed equally to this work.
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Communication
Disaccharides as Sialic Acid Aldolase Substrates: Synthesis of Disaccharides Containing a Sialic Acid at the Reducing End†
Article first published online: 19 FEB 2007
DOI: 10.1002/anie.200604799
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Huang, S., Yu, H. and Chen, X. (2007), Disaccharides as Sialic Acid Aldolase Substrates: Synthesis of Disaccharides Containing a Sialic Acid at the Reducing End. Angew. Chem. Int. Ed., 46: 2249–2253. doi: 10.1002/anie.200604799
- †
This work was supported by a National Sciences Foundation CAREER Award (grant no.: CHE-0548235) and start-up funds from the Regents of the University of California.
Publication History
- Issue published online: 9 MAR 2007
- Article first published online: 19 FEB 2007
- Manuscript Received: 27 NOV 2006
Funded by
- National Sciences Foundation. Grant Number: CHE-0548235
Keywords:
- aldolases;
- carbohydrates;
- chemoenzymatic synthesis;
- enzyme catalysis;
- sialic acids
Synthesis with an unfussy enzyme: Escherichia coli sialic acid aldolase has been found to be unusually flexible in accepting disaccharides containing a mannose (Man) or N-glycolylmannosamine (ManNGc) at the reducing end as substrates for synthesizing disaccharides containing deaminoneuraminic acid (KDN)or N-glycolylneuraminic acid (Neu5Gc) at the reducing end (see scheme; Gal: galactose; Glc: glucose). The chemoenzymatic method is general and efficient.