These authors contributed equally to this work.
Disaccharides as Sialic Acid Aldolase Substrates: Synthesis of Disaccharides Containing a Sialic Acid at the Reducing End†
Article first published online: 19 FEB 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 46, Issue 13, pages 2249–2253, March 19, 2007
How to Cite
Huang, S., Yu, H. and Chen, X. (2007), Disaccharides as Sialic Acid Aldolase Substrates: Synthesis of Disaccharides Containing a Sialic Acid at the Reducing End. Angew. Chem. Int. Ed., 46: 2249–2253. doi: 10.1002/anie.200604799
This work was supported by a National Sciences Foundation CAREER Award (grant no.: CHE-0548235) and start-up funds from the Regents of the University of California.
- Issue published online: 9 MAR 2007
- Article first published online: 19 FEB 2007
- Manuscript Received: 27 NOV 2006
- National Sciences Foundation. Grant Number: CHE-0548235
- chemoenzymatic synthesis;
- enzyme catalysis;
- sialic acids
Synthesis with an unfussy enzyme: Escherichia coli sialic acid aldolase has been found to be unusually flexible in accepting disaccharides containing a mannose (Man) or N-glycolylmannosamine (ManNGc) at the reducing end as substrates for synthesizing disaccharides containing deaminoneuraminic acid (KDN)or N-glycolylneuraminic acid (Neu5Gc) at the reducing end (see scheme; Gal: galactose; Glc: glucose). The chemoenzymatic method is general and efficient.