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Keywords:

  • aldolases;
  • carbohydrates;
  • chemoenzymatic synthesis;
  • enzyme catalysis;
  • sialic acids
Thumbnail image of graphical abstract

Synthesis with an unfussy enzyme: Escherichia coli sialic acid aldolase has been found to be unusually flexible in accepting disaccharides containing a mannose (Man) or N-glycolylmannosamine (ManNGc) at the reducing end as substrates for synthesizing disaccharides containing deaminoneuraminic acid (KDN)or N-glycolylneuraminic acid (Neu5Gc) at the reducing end (see scheme; Gal: galactose; Glc: glucose). The chemoenzymatic method is general and efficient.