Dynamic Hydrogels: Translating a Protein Conformational Change into Macroscopic Motion

Authors

  • William L. Murphy Dr.,

    1. Department of Chemistry, Howard Hughes Medical Institute, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-1677
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  • W. Shannon Dillmore Dr.,

    1. Department of Chemistry, Howard Hughes Medical Institute, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-1677
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  • Justin Modica,

    1. Department of Chemistry, Howard Hughes Medical Institute, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-1677
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  • Milan Mrksich Prof.

    1. Department of Chemistry, Howard Hughes Medical Institute, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-1677
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  • This research was supported by the NSF-supported Materials Research Science & Engineering Center. The authors thank Wei-Jen Tang for providing engineered CaM plasmids and Young-Sam Lee for technical assistance.

Abstract

original image

Natural inspiration: A bioinspired functional material in the form of a hydrogel created by cross-linking an engineered version of calmodulin, a protein which undergoes a conformational change in response to ligand binding (see schematic representation), with a four-armed poly(ethylene glycol) molecule terminated with acrylate groups decreased in volume by 20 % when treated with the ligand trifluoperazine. Multiple cycles of gel swelling and shrinkage were possible.

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