DNA Photography: An Ultrasensitive DNA-Detection Method Based on Photographic Techniques

Authors

  • David M. Hammond Dr.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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    • These authors contributed equally to the work.

  • Antonio Manetto Dr.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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    • These authors contributed equally to the work.

  • Johannes Gierlich Dipl.-Chem.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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  • Vladimir A. Azov Dr.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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  • Philipp M. E. Gramlich Dipl.-Chem.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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  • Glenn A. Burley Dr.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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  • Melanie Maul Dipl.-Ing.,

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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  • Thomas Carell Prof. Dr.

    1. Fakultät für Chemie und Pharmazie, Ludwigs-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München, Germany, Fax: (+49) 89-2180-77756
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  • We thank Martin Hadden of Ilford and Dr. Peter Bergthaller and Dr. Klaus Wagner for helpful discussion, and the Volkswagen Foundation, the Deutsche Forschungsgemeinschaft, and BASF AG for financial support. A.M. thanks the EU Marie Curie Training and Mobility Program under contract MRTN-CT-2003–505086 [CLUSTOXDNA] for an ESR fellowship. J.G. and P.M.E.G. thank the Fonds of the Chemical Industry and Cusanuswerk, respectively, for predoctoral fellowships. G.A.B. thanks the Alexander von Humboldt Foundation for a research fellowship.

Abstract

original image

All there in black and white: DNA labeled with spectral sensitizers applied to commercially available photopaper allows the detection of oligonucleotides with extreme sensitivity. The detection of the DNA with the help of black and white photography is shown in the picture (ODN=oligodeoxyribonucleotide).

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