We thank Prof. Dr. Isamu Matsunaga for his kind gift of the expression system of P450BSβ. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas Chemistry of Coordination Space Grant 16074208 (to Y.W. and Y.S.) from the Ministry of Education, Culture, Sports, Science, and Technology (Japan). O.S. is supported by JSPS Research Fellowships for Young Scientists.
Hydrogen Peroxide Dependent Monooxygenations by Tricking the Substrate Recognition of Cytochrome P450BSβ†
Article first published online: 27 MAR 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 46, Issue 20, pages 3656–3659, May 11, 2007
How to Cite
Shoji, O., Fujishiro, T., Nakajima, H., Kim, M., Nagano, S., Shiro, Y. and Watanabe, Y. (2007), Hydrogen Peroxide Dependent Monooxygenations by Tricking the Substrate Recognition of Cytochrome P450BSβ. Angew. Chem. Int. Ed., 46: 3656–3659. doi: 10.1002/anie.200700068
- Issue published online: 4 MAY 2007
- Article first published online: 27 MAR 2007
- Manuscript Received: 6 JAN 2007
- Grants-in-Aid for Scientific Research on Priority Areas Chemistry of Coordination Space. Grant Number: 16074208
- cytochrome P450;
- enzyme catalysis;
- hydrogen peroxide;
Trick or treat: Cytochrome P450BSβ was transformed into a monooxygenase suitable for practical use by employing a simple substrate trick. The substrate specificity of P450BSβ was altered drastically by a decoy molecule, while its intrinsic advantage, the use of hydrogen peroxide, was retained. The catalytic activities and the enantioselectivity of the H2O2–P450BSβ system are highly dependent on the structure of the decoy molecule.