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Triplex Molecular Beacons as Modular Probes for DNA Detection

Authors

  • Tom N. Grossmann Dipl.-Chem.,

    1. Institut für Chemie der Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany, Fax: (+49) 30-2093-7266
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    • Both authors contributed equally to this work.

  • Lars Röglin Dipl.-Chem.,

    1. Institut für Chemie der Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany, Fax: (+49) 30-2093-7266
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    • Both authors contributed equally to this work.

  • Oliver Seitz Prof. Dr.

    1. Institut für Chemie der Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany, Fax: (+49) 30-2093-7266
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  • We acknowledge support from Schering AG. T.N.G. is grateful for a fellowship from the Studienstiftung des deutschen Volkes.

Abstract

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Reporting live: Triplex formation is used to construct a stem–loop probe, which is opened upon binding of the DNA target (red). The triplex molecular beacon is composed of a single DNA strand (blue), a stem-forming oligomer (black), and is labeled with a fluorophore and a quencher (red and blue circles). This concept facilitates the introduction of further functionalities such as additional quenchers in the assembly of “superquenched” beacons.

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