A Pocket-Sized Convective PCR Thermocycler

Authors

  • Nitin Agrawal Dr.,

    1. Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979-458-1002
    2. Present address: Center for Engineering in Medicine/Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital and Harvard Medical School, Boston, MA 02114, USA
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  • Yassin A. Hassan Prof.,

    1. Department of Nuclear Engineering, Texas A&M University, College Station, TX 77843, USA
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  • Victor M. Ugaz Prof.

    1. Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979-458-1002
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  • This work was supported by the National Institutes of Health under grants K22-HG02297 and R01-HG003364. We thank Prof. Mihir Sen for helpful discussions regarding modeling the convective flow. We are also grateful to Ling Zhen for assistance with computational-flow simulations.

Abstract

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Is that a PCR in your pocket? An innovative thermocycling system is presented that harnesses natural convection to perform rapid multiplex and long-target DNA amplification by the polymerase chain reaction (PCR). The design is inherently simple and consumes minimal electrical power, enabling a pocket-sized battery-powered device to be constructed at a cost of approximately US$10.

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