We thank Dr. E. Pierson for guidance with the fluorescence microscope and NWO-ACTS and Synthon B.V. for financial support.
Positional Assembly of Enzymes in Polymersome Nanoreactors for Cascade Reactions†
Article first published online: 17 AUG 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 46, Issue 39, pages 7378–7382, October 1, 2007
How to Cite
Vriezema, Dennis M., Garcia, Paula M. L., Sancho Oltra, N., Hatzakis, Nikos S., Kuiper, Suzanne M., Nolte, Roeland J. M., Rowan, Alan E. and van Hest, Jan C. M. (2007), Positional Assembly of Enzymes in Polymersome Nanoreactors for Cascade Reactions. Angew. Chem. Int. Ed., 46: 7378–7382. doi: 10.1002/anie.200701125
- Issue published online: 20 SEP 2007
- Article first published online: 17 AUG 2007
- Manuscript Revised: 18 APR 2007
- Manuscript Received: 14 MAR 2007
- Synthon B.V.
- cascade reactions;
- supramolecular chemistry
In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes. A one-pot multistep reaction sequence is performed with the nanoreactor in combination with free Candida antarctica lipase B (CALB) in the bulk solution (see picture; ABTS: 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)).