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Positional Assembly of Enzymes in Polymersome Nanoreactors for Cascade Reactions


  • We thank Dr. E. Pierson for guidance with the fluorescence microscope and NWO-ACTS and Synthon B.V. for financial support.


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In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes. A one-pot multistep reaction sequence is performed with the nanoreactor in combination with free Candida antarctica lipase B (CALB) in the bulk solution (see picture; ABTS: 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)).

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