Nitroxylcob(III)alamin: Synthesis and X-ray Structural Characterization

Authors

  • Luciana Hannibal,

    1. Department of Chemistry and School of Biomedical Sciences, Kent State University, Kent, OH 44242, USA, Fax: (+1) 330-672-3816
    2. Department of Cell Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
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  • Clyde A. Smith Dr.,

    1. Stanford Synchrotron Radiation Laboratory, Stanford University, Menlo Park, CA 94025, USA
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  • Donald W. Jacobsen Dr.,

    1. Department of Cell Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    2. Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
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  • Nicola E. Brasch Dr.

    1. Department of Chemistry and School of Biomedical Sciences, Kent State University, Kent, OH 44242, USA, Fax: (+1) 330-672-3816
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  • This research was funded by Kent State University (N.E.B. and L.H.) and the National Heart Lung and Blood Institute of the National Institutes of Health (HL71907) (D.W.J.).

Abstract

original image

Mystery solved: The long-elusive crystal structure of nitrosylcobalamin (NOCbl) reveals that the Co-N-O angle is 117.4–121.4°; hence, NOCbl is best described as nitroxylcob(III)alamin in the solid state (see picture: Co purple, N blue, O red, P orange, C gray, H white). The length of the Co[BOND]N bond trans to the NO ligand is typical of those seen when strong β-axial ligands are positioned trans to the 5,6-dimethylbenzimidazole group.

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