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A Microarray Strategy for Mapping the Substrate Specificity of Protein Tyrosine Phosphatase

Authors

  • Maja Köhn Dr.,

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • Marta Gutierrez-Rodriguez Dr.,

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • Pascal Jonkheijm Dr.,

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • Stefan Wetzel Dipl.-Chem.,

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • Ron Wacker Dr.,

    1. Chimera Biotec GmbH, Emil-Figge-Strasse 76a, 44227 Dortmund, Germany
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  • Hendrik Schroeder Dr.,

    1. Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Universität Dortmund, Otto-Hahn Strasse 6, 44227 Dortmund, Germany
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  • Heino Prinz Priv.-Doz.,

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • Christof M. Niemeyer Prof.,

    1. Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Universität Dortmund, Otto-Hahn Strasse 6, 44227 Dortmund, Germany
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  • Rolf Breinbauer Prof.,

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • Stefan E. Szedlacsek Prof.,

    1. Department of Enzymology, Institute of Biochemistry, Splaiul Independentei 296, 060031 Bucharest, Romania
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  • Herbert Waldmann Prof.

    1. Department of Chemical Biology, Max-Planck Institute für molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    2. Fachbereich Chemie, Organische Chemie, Universität Dortmund, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
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  • This work was supported by the Max-Planck Gesellschaft, the research program “Molecular Basics of Biosciences” of the University of Dortmund, the Fonds der Chemischen Industrie, and the Zentrum für Angewandte Chemische Genomik. P.J. thanks the Alexander von Humboldt Stiftung and S.W. thanks Novartis for a scholarship.

Abstract

original image

Which substrate will it be? Phosphotyrosine peptide microarrays have allowed the substrate specificity to be mapped for two prototypical protein-tyrosine phosphatases (PTPs): PTP1B and PTPμ. The knowledge gained was used for molecular docking studies (see picture: the docking of a peptide in the binding pocket of PTPμ) and the design of an inhibitor for PTPμ.

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