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Continuous Fluorometric Assays for Acetylcholinesterase Activity and Inhibition with Conjugated Polyelectrolytes

Authors

  • Fude Feng,

    1. National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P.R. China, Fax: (+86) 10-6263-6680
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  • Yanli Tang,

    1. National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P.R. China, Fax: (+86) 10-6263-6680
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  • Shu Wang Prof.,

    1. National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P.R. China, Fax: (+86) 10-6263-6680
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  • Yuliang Li Prof.,

    1. National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P.R. China, Fax: (+86) 10-6263-6680
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  • Daoben Zhu Prof.

    1. National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P.R. China, Fax: (+86) 10-6263-6680
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  • We are grateful for the financial support of the “100 Talents” program of the Chinese Academy of Sciences, the National Natural Science Foundation of China (20601027, 20574073, and 20421101), the National Basic Research Program of China (No.2006CB806200), and the Major Research Plan of China (No. 2006CB932100).

Abstract

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Quick but sensitive: A super-quenched fluorogenic complex of a cationic acetylcholine (ACh) derivative with an energy-acceptor tag and an anionic water-soluble conjugated polymer forms the basis of a highly effective fluorescence turn-on assay for studying the enzyme kinetics and inhibition of acetylcholinesterase (AChE; see schematic representation of the assay). FRET=fluorescence resonant energy transfer.

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