Photochromic Rhodamines Provide Nanoscopy with Optical Sectioning

Authors


  • This work was supported by the European Commission through a SPOTLITE grant to S.W.H. and a Marie Curie Fellowship to M.B., and a BMBF program (Biophotonics) grant. We thank C. Geisler and B. Rankin for helpful assistance.

Abstract

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Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed.

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