Rational Design of a Fluorescence-Turn-On Sensor Array for Phosphates in Blood Serum

Authors

  • Grigory V. Zyryanov,

    1. Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH 43403, USA, Fax: (+1) 419-372-9809
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  • Manuel A. Palacios,

    1. Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH 43403, USA, Fax: (+1) 419-372-9809
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  • Pavel Anzenbacher Jr. Prof. Dr.

    1. Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH 43403, USA, Fax: (+1) 419-372-9809
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  • P.A. acknowledges support from the Alfred P. Sloan Foundation, BGSU (Technology Innovations Enhancement grant), and the NSF (SENSOR no. 0330267). M.A.P. acknowledges support from the McMaster Endowment. We would like to thank Prof. Franz P. Schmidtchen for helpful discussions during the preparation of this manuscript.

Abstract

original image

Getting turned on: An order-of-magnitude fluorescence amplification of simple tripodal sensors has been observed in the presence of phosphate ions. An X-ray structure analysis of one of the complexes (see picture) shows the binding of three phosphate ions, which closely resembles the anionic part of ATP, by the sensor. The sensors were used to generate cross-reactive arrays that can detect anions in human serum.

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