Get access

Reverse Glycoblotting Allows Rapid-Enrichment Glycoproteomics of Biopharmaceuticals and Disease-Related Biomarkers

Authors

  • Masaki Kurogochi Dr.,

    1. Division of Advanced Life Science, Graduate School of Advanced Life Science, Frontier Research Center for the Post-Genomic Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
    Search for more papers by this author
  • Maho Amano Dr.,

    1. Division of Advanced Life Science, Graduate School of Advanced Life Science, Frontier Research Center for the Post-Genomic Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
    Search for more papers by this author
  • Masataka Fumoto Dr.,

    1. Shionogi & Co. Ltd., Osaka 553–0002, Japan
    Search for more papers by this author
  • Akio Takimoto Dr.,

    1. Shionogi & Co. Ltd., Osaka 553–0002, Japan
    Search for more papers by this author
  • Hirosato Kondo Dr.,

    1. Shionogi & Co. Ltd., Osaka 553–0002, Japan
    Search for more papers by this author
  • Shin-Ichiro Nishimura Prof. Dr.

    1. Division of Advanced Life Science, Graduate School of Advanced Life Science, Frontier Research Center for the Post-Genomic Science and Technology, Hokkaido University, N21, W11, Sapporo 001-0021, Japan, Fax: (+81) 11-706-9042
    2. Drug-Seeds Discovery Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan
    Search for more papers by this author

  • This work was partly supported by funding from the Japan Science and Technology Agency (JST) for “Development of system and technology for advanced measurement and analysis (SENTAN)” and for “Innovation COE Program for Future Drug Discovery and Medical Care”. We are grateful to Dr. Y. Shinohara, Dr. Y. Miura, and Dr. H. Nakagawa for valuable suggestions and discussions on mouse serum N-glycan profiling.

Abstract

original image

Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).

Ancillary