We would like to thank Dr. Kara Herlihy, Dr. Ralph Mazitschek, Dr. Carlos Tassa, Jason Fuller, Dr. Steve Haggarty, Dr. Jianping Cui, Dr. Letian Kuai, Dr. Marvin Yu, and Dr. Philip Yeske (Fluorous Technologies) for reagents or comments. Work described herein has been funded in whole or in part with Federal funds from the National Cancer Institute's Initiative for Chemical Genetics, National Institutes of Health, under contract no. N01-CO-12400.
Fluorous-Based Small-Molecule Microarrays for the Discovery of Histone Deacetylase Inhibitors†
Article first published online: 17 SEP 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 46, Issue 42, pages 7960–7964, October 22, 2007
How to Cite
Vegas, Arturo J., Bradner, James E., Tang, W., McPherson, Olivia M., Greenberg, Edward F., Koehler, Angela N. and Schreiber, Stuart L. (2007), Fluorous-Based Small-Molecule Microarrays for the Discovery of Histone Deacetylase Inhibitors. Angew. Chem. Int. Ed., 46: 7960–7964. doi: 10.1002/anie.200703198
- Issue published online: 12 OCT 2007
- Article first published online: 17 SEP 2007
- Manuscript Received: 17 JUL 2007
- National Cancer Institute's Initiative for Chemical Genetics
- National Institutes of Health. Grant Number: N01-CO-12400
- fluorous tags;
- high-throughput screening;
Noncovalent immobilization is an attractive method for identifying inhibitors of histone deacetylases (HDACs). Fluorous-based small-molecule microarrays were validated as an effective method. Three enzymes and three assays (microarray, biochemical activity, and surface plasmon resonance) were used to identify inhibitors of HDACs and to compare them.