Allosteric Activation of HtrA Protease DegP by Stress Signals during Bacterial Protein Quality Control


  • This work was supported by Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie and BBSRC (M.E.), Austrian Science Fund FWF P17881-B10 (T.K.) and by BBSRC (D.J., M.E.)


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Unleashing the proteolytic activity of DegP: A study with synthetic mimics of cellular stress signals reveals an allosteric (chemical) activation mechanism of the bacterial HtrA protease DegP, leading to a fine-tuned amplification of protein degradation during bacterial protein quality control (see scheme: binding of a peptide sequence (circles) increases activation. The nature of the penultimate residue (red) is shown to be decisive).