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3D Nanoscopy: Bringing Biological Nanostructures into Sharp Focus

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Abstract

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Zooming in: Ultrahigh-resolution microscopy is developing rapidly by different approaches. Photoactivation–localization microscopy (PALM) can now provide 3D images with fast acquisition times through the use of photoactivatable rhodamines and two-photon excitation. In PALM, individual emitters can be localized with nanometer precision simply by determining the centroid of the emission distribution.

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