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Peptide Microarray for High-Throughput Determination of Phosphatase Specificity and Biology

Authors

  • Hongyan Sun,

    1. Departments of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore, Fax: (+65) 6779-1691
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  • Candy H. S. Lu,

    1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
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  • Mahesh Uttamchandani,

    1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
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  • Yun Xia,

    1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
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  • Yih-Cherng Liou Prof. Dr.,

    1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
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  • Shao Q. Yao Prof. Dr.

    1. Departments of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore, Fax: (+65) 6779-1691
    2. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
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  • Funding support was provided by the National University of Singapore (NUS) and the Agency for Science, Technology and Research (A*STAR) of Singapore.

Abstract

original image

Identifying phosphatase substrates: A peptide microarray has been developed for the high-throughput study of Ser/Thr phosphatases. Putative peptide substrates, upon immobilization onto a glass slide, could be used to obtain kinetic information and identify the substrate preferences of a Ser/Thr phosphatase (see schematic representation); with this information, new biology of the enzyme could be discovered.

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