This work was supported by the NIH (DK073368), the American Heart Association, W.M. Keck Center, the Young Clinical Scientist Award Program of the Flight Attendant Medical Research Institute, and 3M (to J.Z.).
A Tunable FRET Circuit for Engineering Fluorescent Biosensors†
Article first published online: 4 DEC 2007
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 3, pages 500–502, January 4, 2008
How to Cite
Allen, Michael D. and Zhang, J. (2008), A Tunable FRET Circuit for Engineering Fluorescent Biosensors. Angew. Chem. Int. Ed., 47: 500–502. doi: 10.1002/anie.200703493
- Issue published online: 21 DEC 2007
- Article first published online: 4 DEC 2007
- Manuscript Received: 1 AUG 2007
- FRET (fluorescence resonant energy transfer);
Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.