Diazirine-Based DNA Photo-Cross-Linking Probes for the Study of Protein–DNA Interactions

Authors

  • Uddhav Kumar Shigdel,

    1. Department of Chemistry, University of Chicago, 5735 South Ellis Avenue Chicago, IL 60637, USA, Fax: (+1) 773-702-0805
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  • Junliang Zhang Dr.,

    1. Department of Chemistry, University of Chicago, 5735 South Ellis Avenue Chicago, IL 60637, USA, Fax: (+1) 773-702-0805
    2. Current address: Department of Chemistry, East China Normal University, Shanghai, P.R. China
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  • Chuan He Prof.

    1. Department of Chemistry, University of Chicago, 5735 South Ellis Avenue Chicago, IL 60637, USA, Fax: (+1) 773-702-0805
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  • This research was supported by the W. M. Keck Foundation, the Arnold and Mabel Beckman Foundation, the Camille & Henry Dreyfus Foundation, the Research Corporation, and a Concept Award from the U.S. Army Medical Research Office.

Abstract

original image

Small modification, large effect: Diazirine can be installed quickly and simply in the major or minor groove of DNA for efficient photo-cross-linking to proteins (see picture). This method should be suitable for mapping out protein–DNA interactions, in particular those that may be sensitive to steric hindrance.

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