Communication

Addition of an α-Hydroxy Acid to the Genetic Code of Bacteria†

  1. Jiantao Guo1,‡,
  2. Jiangyun Wang1,‡,
  3. J. Christopher Anderson Prof.1,2,
  4. Peter G. Schultz Prof.1

Article first published online: 11 DEC 2007

DOI: 10.1002/anie.200704074

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 47, Issue 4, pages 722–725, January 11, 2008

Additional Information

How to Cite

Guo, J., Wang, J., Anderson, J. Christopher. and Schultz, Peter G. (2008), Addition of an α-Hydroxy Acid to the Genetic Code of Bacteria. Angew. Chem. Int. Ed., 47: 722–725. doi: 10.1002/anie.200704074

Author Information

  1. 1

    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440

  2. 2

    Department of Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720-1762, USA

  1. ‡

    These authors contributed equally to this work.

  1. †

    We thank Bill Webb for help in protein mass spectrometry, and we are grateful to the DOE (ER46051) and the Skaggs Institute for Chemical Biology for support. We thank Dr. M. Sever and Dr. W. Liu for helpful discussion.

Publication History

  1. Issue published online: 8 JAN 2008
  2. Article first published online: 11 DEC 2007
  3. Manuscript Revised: 11 NOV 2007
  4. Manuscript Received: 4 SEP 2007

Funded by

  • DOE. Grant Number: ER46051
  • Skaggs Institute for Chemical Biology

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Keywords:

  • α-hydroxy acids;
  • backbone mutations;
  • biosynthesis;
  • proteins
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Playing tag: The α-hydroxy acid p-hydroxy-L-phenyllactic acid has been genetically incorporated into proteins in E. coli in response to an amber nonsense codon. The site-specific introduction of backbone ester mutations was used for the site-specific hydrolysis of an affinity tag, as well as for determination of the energetic contributions of backbone hydrogen bonds to protein stability.

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