Communication

Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity†

  1. Carole Baffert Dr.1,
  2. Marie Demuez Dr.2,
  3. Laurent Cournac Dr.3,
  4. Bénédicte Burlat Dr.1,
  5. Bruno Guigliarelli Prof.1,
  6. Patrick Bertrand Prof.1,
  7. Laurence Girbal Dr.2,
  8. Christophe Léger Dr.1

Article first published online: 1 FEB 2008

DOI: 10.1002/anie.200704313

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 47, Issue 11, pages 2052–2054, February 28, 2008

Additional Information

How to Cite

Baffert, C., Demuez, M., Cournac, L., Burlat, B., Guigliarelli, B., Bertrand, P., Girbal, L. and Léger, C. (2008), Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity. Angew. Chem. Int. Ed., 47: 2052–2054. doi: 10.1002/anie.200704313

Author Information

  1. 1

    Laboratoire de Bioénergétique et Ingénierie des Protéines, CNRS, Université de Provence, Marseille, France, Fax: (+33) 4-9116-4578 http://bip.cnrs-mrs.fr/bip06/

  2. 2

    Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, Toulouse, France

  3. 3

    Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA, DSV, IBEB, CNRS, Univ. Aix Marseille II, St Paul-lez-Durance, France

  1. †

    We acknowledge Sébastien Dementin for help and fruitful discussions, funding from the ANR, and support from the Pôle de Compétitivité Capénergies.

Publication History

  1. Issue published online: 22 FEB 2008
  2. Article first published online: 1 FEB 2008
  3. Manuscript Revised: 21 NOV 2007
  4. Manuscript Received: 18 SEP 2007

Funded by

  • ANR
  • Pôle de Compétitivité Capénergies

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Keywords:

  • electron transfer;
  • hydrogenases;
  • inhibition;
  • kinetics;
  • protein film voltammetry
Thumbnail image of graphical abstract

Surprisingly uninhibited: The inhibition of hydrogenases by oxygen is intensely studied because this is the main obstacle to using these enzymes in biofuel cells. The hydrogenase from Clostridium acetobutylicum (see structure) was found to react surprisingly slowly with O2. The inhibition mechanism was elucidated and the kinetics were quantitatively defined. This is a prerequisite for improving the enzyme further by genetic engineering and for assessing its potential in technological devices.

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