This research was supported by grants from the National Institutes of Health to T.P.B. (GM069618). We would like to thank Prof. Frank C. Schroeder for helpful discussions regarding the NMR characterization. Pdx1=pyridoxal phosphate synthase.
1521-3773/asset/2002_left.gif?v=1&s=ac6b0d94a94d7ce7a210002b8096b42feffc0bcf)
1521-3773/asset/2002_right.gif?v=1&s=451042aa3415ae3ad0729984d26dee1866aca82e)
Communication
Trapping of a Chromophoric Intermediate in the Pdx1-Catalyzed Biosynthesis of Pyridoxal 5′-Phosphate†
Article first published online: 7 FEB 2008
DOI: 10.1002/anie.200704390
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 47, Issue 11, pages 2102–2105, February 28, 2008
Additional Information
How to Cite
Hanes, Jeremiah W., Keresztes, I. and Begley, Tadhg P. (2008), Trapping of a Chromophoric Intermediate in the Pdx1-Catalyzed Biosynthesis of Pyridoxal 5′-Phosphate. Angew. Chem. Int. Ed., 47: 2102–2105. doi: 10.1002/anie.200704390
- †
Publication History
- Issue published online: 22 FEB 2008
- Article first published online: 7 FEB 2008
- Manuscript Received: 23 SEP 2007
Funded by
- National Institutes of Health. Grant Number: GM069618
Keywords:
- biosynthesis;
- intermediates;
- phosphines;
- pyridoxal;
- vitamin B6
I320has it: The serendipitous trapping, by aromatization, of an enzyme-bound chromophoric intermediate (I320; see picture, blue box) involved in vitamin B6 formation using a common biological reducing agent provides new information regarding the structure of the chromophoric species.