Get access

A Bioluminescent Molecular Switch For Glucose


  • This work was supported in part by grants from the National Institutes of Health and the National Aeronautics and Space Administration. We thank the Office of the Vice President of Research at the University of Kentucky for a University Research Professorship to S.D. K.T. acknowledges support from a Gill Fellowship, and S.D. from a Gill Eminent Professorship.


original image

Glowing after a sugar rush: A molecular switch is formed by inserting glucose-binding protein (GBP) into aequorin (AEQ; see picture). This switch is triggered by recognition of glucose by the GBP. For the first time, AEQ is rationally split into two fragments that, upon a molecular recognition event, come together and emit bioluminescence.