We acknowledge helpful discussions with S. Gruner, J. J. Cheng, and A. Garcia. The work is supported in part by the National Science Foundation (NSF) Division of Materials Research (Grant no.: 0409769), the NSF Nanoscale Science and Engineering Centers, and the National Institutes of Health (Grant no.: 1R21K6843-01). The X-ray diffraction data were collected at the Stanford Synchrotron Radiation Laboratory (Palo Alto, CA; BL4-2) and the Advanced Photon Source (Argonne, IL; BESSRCAT BL-12ID).
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Communication
HIV TAT Forms Pores in Membranes by Inducing Saddle-Splay Curvature: Potential Role of Bidentate Hydrogen Bonding†
Article first published online: 12 MAR 2008
DOI: 10.1002/anie.200704444
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Mishra, A., Gordon, Vernita D., Yang, L., Coridan, R. and Wong, Gerard C. L. (2008), HIV TAT Forms Pores in Membranes by Inducing Saddle-Splay Curvature: Potential Role of Bidentate Hydrogen Bonding. Angew. Chem. Int. Ed., 47: 2986–2989. doi: 10.1002/anie.200704444
- †
Publication History
- Issue published online: 1 APR 2008
- Article first published online: 12 MAR 2008
- Manuscript Revised: 8 NOV 2007
- Manuscript Received: 27 SEP 2007
Funded by
- National Science Foundation (NSF) Division of Materials Research. Grant Number: 0409769
- NSF Nanoscale Science and Engineering Centers
- National Institutes of Health
- 1R21K6843-01
Keywords:
- lipids;
- membranes;
- peptides;
- pore formation;
- transduction
Pore performance: The TAT protein of HIV can cross cell membranes with remarkable efficiency. By applying ideas from coordination chemistry, soft-condensed-matter physics, and differential geometry, it has been shown that TAT induces saddle-splay curvature in cell membranes, a process that is required for pore formation (see picture of two nonintersecting networks of pores). The results have potential implications for the design of cell-penetrating peptides.