This work was supported by the Zentrum für Angewandte Chemische Genomik, a joint research initiative founded by the European Union and the Ministry of Innovation and Research of the state Northrhine Westfalia and the Deutsche Forschungsgemeinschaft (grant FE 943/1-1 to U.F.).
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Communication
High-Throughput, Real-Time Monitoring of the Self-Assembly of DNA Nanostructures by FRET Spectroscopy†
Article first published online: 7 FEB 2008
DOI: 10.1002/anie.200704836
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 47, Issue 11, pages 2135–2137, February 28, 2008
Additional Information
How to Cite
Saccà, B., Meyer, R., Feldkamp, U., Schroeder, H. and Niemeyer, Christof M. (2008), High-Throughput, Real-Time Monitoring of the Self-Assembly of DNA Nanostructures by FRET Spectroscopy. Angew. Chem. Int. Ed., 47: 2135–2137. doi: 10.1002/anie.200704836
- †
Publication History
- Issue published online: 22 FEB 2008
- Article first published online: 7 FEB 2008
- Manuscript Revised: 3 DEC 2007
- Manuscript Received: 18 OCT 2007
Funded by
- European Union
- Ministry of Innovation and Research of the state Northrhine Westfalia
- Deutsche Forschungsgemeinschaft. Grant Number: FE 943/1-1
Keywords:
- DNA nanostructures;
- FRET (Förster resonance energy transfer);
- nanobiotechnology;
- self-assembly;
- thermal analysis
Tiles and arrays: The self-assembly of DNA nanostructures can be monitored in real time and with high throughput by FRET spectroscopy. A van't Hoff analysis of the fluorescence data allows for a complete thermodynamic characterization of the tile formation as well as array formation (see picture).