The authors thank Dr. Nicholas Agard and Prof. Isaac Carrico for the mDHFR samples, Dr. Jennifer Prescher for Ac4ManNAz, Prof. Christopher Chang for the use of the fluorimeter, and Dr. Jennifer Czlapinski, Pamela Chang, Jeremy Baskin, and Dr. Christopher de Graffenried for helpful advice and discussion. This work was supported by a NDSEG Fellowship (to M.J.H.) and NIH grant GM058867.
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Communication
A FRET-Based Fluorogenic Phosphine for Live-Cell Imaging with the Staudinger Ligation†
Article first published online: 27 FEB 2008
DOI: 10.1002/anie.200704847
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Hangauer, M. and Bertozzi, C. (2008), A FRET-Based Fluorogenic Phosphine for Live-Cell Imaging with the Staudinger Ligation. Angewandte Chemie International Edition, 47: 2394–2397. doi: 10.1002/anie.200704847
- †
Publication History
- Issue published online: 10 MAR 2008
- Article first published online: 27 FEB 2008
- Manuscript Received: 18 OCT 2007
Funded by
- NIH. Grant Number: GM058867
Keywords:
- azides;
- fluorescent probes;
- glycosylation;
- phosphines;
- Staudinger ligation
Graphical Abstract
Azide imaging: A fluorogenic phosphine based on a FRET-quenching mechanism allows for live-cell imaging of azido sugars by the Staudinger ligation. This design strategy can accommodate numerous fluorophores and complementary quenchers, enabling extension to multicolor imaging. In the image shown, the nuclei are blue while the cell surfaces and Golgi are green.