A FRET-Based Fluorogenic Phosphine for Live-Cell Imaging with the Staudinger Ligation

Authors

  • Matthew J. Hangauer,

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA, Fax: (+1) 510-643-2628
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  • Carolyn R. Bertozzi Prof.

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA, Fax: (+1) 510-643-2628
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  • The authors thank Dr. Nicholas Agard and Prof. Isaac Carrico for the mDHFR samples, Dr. Jennifer Prescher for Ac4ManNAz, Prof. Christopher Chang for the use of the fluorimeter, and Dr. Jennifer Czlapinski, Pamela Chang, Jeremy Baskin, and Dr. Christopher de Graffenried for helpful advice and discussion. This work was supported by a NDSEG Fellowship (to M.J.H.) and NIH grant GM058867.

Abstract

original image

Azide imaging: A fluorogenic phosphine based on a FRET-quenching mechanism allows for live-cell imaging of azido sugars by the Staudinger ligation. This design strategy can accommodate numerous fluorophores and complementary quenchers, enabling extension to multicolor imaging. In the image shown, the nuclei are blue while the cell surfaces and Golgi are green.

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