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Phototriggering of Cell Adhesion by Caged Cyclic RGD Peptides

Authors

  • Svea Petersen,

    1. Max-Planck-Institut für Metallforschung, Heisenbergstrasse 3, 70569 Stuttgart, Germany, Fax: (+49) 711-689-3412
    2. Laboratoire de Chimie Bioorganique UMR7175 CNRS, Faculté de Pharmacie, Université Louis Pasteur Strasbourg, BP 24, 67401 Illkirch, France
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    • These authors contributed equally to this research.

  • José María Alonso Dr.,

    1. Max-Planck-Institut für Metallforschung, Heisenbergstrasse 3, 70569 Stuttgart, Germany, Fax: (+49) 711-689-3412
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    • These authors contributed equally to this research.

  • Alexandre Specht,

    1. Laboratoire de Chimie Bioorganique UMR7175 CNRS, Faculté de Pharmacie, Université Louis Pasteur Strasbourg, BP 24, 67401 Illkirch, France
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  • Portia Duodu,

    1. Laboratoire de Chimie Bioorganique UMR7175 CNRS, Faculté de Pharmacie, Université Louis Pasteur Strasbourg, BP 24, 67401 Illkirch, France
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  • Maurice Goeldner,

    1. Laboratoire de Chimie Bioorganique UMR7175 CNRS, Faculté de Pharmacie, Université Louis Pasteur Strasbourg, BP 24, 67401 Illkirch, France
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  • Aranzazu del Campo Dr.

    1. Max-Planck-Institut für Metallforschung, Heisenbergstrasse 3, 70569 Stuttgart, Germany, Fax: (+49) 711-689-3412
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  • We thank Neo MPS Laboratories (Strasbourg, France) for synthesizing the peptide, and Alexandra Goldyn, Henriette Ries, and Ralf Kemkemer (research group of Prof. J. Spatz, MPI für Metallforschung, Stuttgart, Germany) for help with the cell experiments. The RGD peptide sequence is Arg-Gly-Asp.

Abstract

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Restrained potential: A caged cyclic peptide attached to a surface is able to trigger cell attachment to the surface with spatiotemporal definition upon exposure to light (λ=351 nm). The peptide shows no integrin-binding activity in its caged form, but mediates cell adhesion effectively after irradiation (see optical microscopy image of cells on a surface irradiated through a mask in bands 100 μm in width).

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