This work is part of the research projects Z4 055 0506 and Z5 004 0507. It was supported by the Grant Agency of the Czech Republic (203/05/0043, 203/07/1195), the Ministry of Education (LC512, LC06035), and Gilead Sciences, Inc. (Foster City, CA, USA).
Aminophenyl- and Nitrophenyl-Labeled Nucleoside Triphosphates: Synthesis, Enzymatic Incorporation, and Electrochemical Detection†
Article first published online: 7 FEB 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 11, pages 2059–2062, February 28, 2008
How to Cite
Cahová, H., Havran, L., Brázdilová, P., Pivoňková, H., Pohl, R., Fojta, M. and Hocek, M. (2008), Aminophenyl- and Nitrophenyl-Labeled Nucleoside Triphosphates: Synthesis, Enzymatic Incorporation, and Electrochemical Detection. Angew. Chem. Int. Ed., 47: 2059–2062. doi: 10.1002/anie.200705088
- Issue published online: 22 FEB 2008
- Article first published online: 7 FEB 2008
- Manuscript Received: 3 NOV 2007
- Grant Agency of the Czech Republic. Grant Numbers: 203/05/0043, 203/07/1195
- Ministry of Education. Grant Numbers: LC512, LC06035
- Gilead Sciences, Inc. (Foster City, CA, USA)
Primer extension is used to incorporate labeled nucleoside triphosphates into oligonucleotides (ONs). Aminophenyl and nitrophenyl modifications serve as electrochemical labels that are detectable by either oxidation or reduction, respectively (see representation of the ON double strand and redox curves). The redox potentials of the labels differ depending on the nucleobase and respond to incorporation into ONs.