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Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes


  • We thank Deutsche Forschungsgemeinschaft for funding through SPP 1170.


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Getting a look in: A high-throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real-time analysis of enantioselectivity by determination of the ratio of green and red single-cell fluorescence.

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