Advertisement

A Highly Selective Luminescent Switch-On Probe for Histidine/Histidine-Rich Proteins and Its Application in Protein Staining

Authors

  • Dik-Lung Ma Dr.,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Wing-Leung Wong Dr.,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Wai-Hong Chung,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Fung-Yi Chan,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Pui-Kin So,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Tat-Shing Lai Dr.,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Zhong-Yuan Zhou,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Yun-Chung Leung Prof.,

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author
  • Kwok-Yin Wong Prof.

    1. Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China, Fax: (+1) 852-2364-9932
    Search for more papers by this author

  • This research was supported by The Hong Kong Polytechnic University and the Area of Excellence Fund of the University Grants Committee (AoE/P-10/01). D.L.M. and W.L.W. acknowledge the award of postdoctoral fellowships administered by the Research Committee of The Hong Kong Polytechnic University. We are grateful to Prof. Chi-Ming Che for supporting this research and for his invaluable suggestions.

Abstract

original image

No destaining required: The luminescence properties of an iridium(III)–2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate–polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules.

Ancillary