This research was supported by the Research Resource Center for Biomedical Complex Carbohydrates (P41-RR-5351). We thank Dr. Heather Flanagan-Steet and Dr. Richard Steet for assistance with confocal microscopy studies.
Visualizing Metabolically Labeled Glycoconjugates of Living Cells by Copper-Free and Fast Huisgen Cycloadditions†
Article first published online: 14 FEB 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 12, pages 2253–2255, March 7, 2008
How to Cite
Ning, X., Guo, J., Wolfert, Margreet A. and Boons, G.-J. (2008), Visualizing Metabolically Labeled Glycoconjugates of Living Cells by Copper-Free and Fast Huisgen Cycloadditions. Angew. Chem. Int. Ed., 47: 2253–2255. doi: 10.1002/anie.200705456
- Issue published online: 3 MAR 2008
- Article first published online: 14 FEB 2008
- Manuscript Received: 29 NOV 2007
- Research Resource Center for Biomedical Complex Carbohydrates. Grant Number: P41-RR-5351
- click chemistry;
CuI-free click: 4-Dibenzocyclooctynol reacts, in the absence of a CuI catalyst, exceptionally fast with azido-containing saccharides and amino acids to give stable triazoles. A biotin-modified derivative is ideally suited for visualizing and tracking glycoconjugates of living cells that are metabolically labeled with azido-containing monosaccharides (see image).