P.M.E.G. thanks the Cusanuswerk for a PhD scholarship. J.G. thanks the Fonds der chemischen Industrie for a PhD scholarship. We thank Dr. G. Clever for the preparation of the anthraquinone azides and pyrene azides. The work was supported by the Fonds der Chemischen Industrie and the Deutsche Forschungsgemeinsschaft (SFB 749 and SFB 486). Support by the Excellence Cluster NIM (Nanoinitiative Munich) is greatly appreciated.
Click–Click–Click: Single to Triple Modification of DNA†
Article first published online: 28 MAR 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 18, pages 3442–3444, April 21, 2008
How to Cite
Gramlich, Philipp M. E., Warncke, S., Gierlich, J. and Carell, T. (2008), Click–Click–Click: Single to Triple Modification of DNA. Angew. Chem. Int. Ed., 47: 3442–3444. doi: 10.1002/anie.200705664
- Issue published online: 14 APR 2008
- Article first published online: 28 MAR 2008
- Manuscript Received: 11 DEC 2007
- Fonds der Chemischen Industrie
- Deutsche Forschungsgemeinsschaft
- click chemistry;
- DNA labeling
One, two, or three: An efficient, modular, and robust protocol has been developed for the multiple functionalization of DNA. It is based on the click reaction of azides with the alkyne substituents on an oligodeoxyribonucleotide (ODN), which was prepared by the standard phosphoramidite method (see scheme). ODNs can thus be labeled with two sensitive molecules, and even triple modification is possible.