Click–Click–Click: Single to Triple Modification of DNA


  • P.M.E.G. thanks the Cusanuswerk for a PhD scholarship. J.G. thanks the Fonds der chemischen Industrie for a PhD scholarship. We thank Dr. G. Clever for the preparation of the anthraquinone azides and pyrene azides. The work was supported by the Fonds der Chemischen Industrie and the Deutsche Forschungsgemeinsschaft (SFB 749 and SFB 486). Support by the Excellence Cluster NIM (Nanoinitiative Munich) is greatly appreciated.


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One, two, or three: An efficient, modular, and robust protocol has been developed for the multiple functionalization of DNA. It is based on the click reaction of azides with the alkyne substituents on an oligodeoxyribonucleotide (ODN), which was prepared by the standard phosphoramidite method (see scheme). ODNs can thus be labeled with two sensitive molecules, and even triple modification is possible.