Q.L. is grateful to the Petroleum Research Fund, University at Buffalo, and New York State Center of Excellence in Bioinformatics and Life Sciences for financial support.
A Photoinducible 1,3-Dipolar Cycloaddition Reaction for Rapid, Selective Modification of Tetrazole-Containing Proteins†
Article first published online: 29 FEB 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 15, pages 2832–2835, March 31, 2008
How to Cite
Song, W., Wang, Y., Qu, J., Madden, Michael M. and Lin, Q. (2008), A Photoinducible 1,3-Dipolar Cycloaddition Reaction for Rapid, Selective Modification of Tetrazole-Containing Proteins. Angew. Chem. Int. Ed., 47: 2832–2835. doi: 10.1002/anie.200705805
- Issue published online: 20 MAR 2008
- Article first published online: 29 FEB 2008
- Manuscript Received: 18 DEC 2007
- Petroleum Research Fund
- University at Buffalo
- New York State Center of Excellence in Bioinformatics and Life Sciences
- dipolar cycloaddition;
Reactive but biologically inert: The bioorthogonal title reaction enables highly specific chemical modifications, such as lipidation, of engineered proteins containing a diphenyltetrazole group (see scheme). The cycloaddition in biological media is extremely fast (≤1 min) and tolerant of proteinaceous groups. Strongly fluorescent pyrazoline cycloadducts are generated with simple alkenes.