This work was supported by a Core Research for Evolutional Science and Technology (CREST) grant from the Japan Science and Technology Agency (JST).
Charge-Conversion Ternary Polyplex with Endosome Disruption Moiety: A Technique for Efficient and Safe Gene Delivery†
Article first published online: 4 JUN 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 28, pages 5163–5166, June 27, 2008
How to Cite
Lee, Y., Miyata, K., Oba, M., Ishii, T., Fukushima, S., Han, M., Koyama, H., Nishiyama, N. and Kataoka, K. (2008), Charge-Conversion Ternary Polyplex with Endosome Disruption Moiety: A Technique for Efficient and Safe Gene Delivery. Angew. Chem. Int. Ed., 47: 5163–5166. doi: 10.1002/anie.200800963
- Issue published online: 20 JUN 2008
- Article first published online: 4 JUN 2008
- Manuscript Revised: 10 APR 2008
- Manuscript Received: 28 FEB 2008
- Japan Science and Technology Agency (JST)
- charge conversion;
- gene delivery;
Wrapped for special delivery: A ternary polyplex, with an endosomal disruption moiety based on the charge-conversion polymer pAsp(DET-Aco), showed negative charges for serum stability and low cytotoxicity, but the charges became positive and the endosome disruption moiety was exposed (see picture). High transfection efficiency and minimal cytotoxicity were observed with primary cells.