Bicelle-Enabled Structural Studies on a Membrane-Associated Cytochrome b5 by Solid-State MAS NMR Spectroscopy


  • This study was supported by research funds from NIH (to A.R. and L.W.), the office of vice president for research (to A.R.), and a VA Merit Review grant to L.W. We thank the 900 MHz Facilities at East Lansing and Tallahassee. MAS=magic angle spinning.


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Spinning bicelles: Solving 3D structures of membrane proteins is a great challenge because of the difficulty in finding well-behaved model membranes. Bicelles are well suited to overcome these difficulties and enable the use of solid-state MAS NMR spectroscopy experiments for studies on a large soluble domain containing a low concentration of membrane protein cytochrome b5 at 37 °C.